Insights into the microbiological and virulence characteristics of bacteria in orthopaedic implant infections: A study from Pakistan

The exponential increase in the prevalence of multidrug resistant bacteria has resulted in limiting surgical treatment options globally, potentially causing biofilm-related complications, implant failure, and severe consequences. This study aims to isolate and characterize bacteria from post-surgical orthopaedic implant infections and screening for multiple antibiotic resistance. A cross-sectional study was conducted, involving isolation of forty-four dominant pathogenic bacterial isolates from 16 infected implant samples from across Islamabad and Rawalpindi. Out of forty-four, 38% cocci and 61% bacilli were obtained. Approximately 90% of isolates showed multiple antibiotic resistance (MAR) index of more than 0.2. Eleven strains were identified via 16S rRNA gene sequencing as Pseudomonas aeruginosa, Bacillus spp., Planococcus chinensis, Staphylococcus, Escherichia coli and Enterobacter cloacae. The bacterial strain E. coli MB641 showed sensitivity to Polymyxin only, and was resistant to all other antibiotics used. Maximum biofilm forming ability 0.532 ± 0.06, 0.55 ± 0.01 and 0.557 ± 0.07 was observed in Pseudomonas aeruginosa MB663, Pseudomonas aeruginosa MB664 and Bacillus spp. MB647 respectively after 24 hours of incubation. EPS production of bacterial strains was assessed, the polysaccharides and protein content of EPS were found to be in the range of 11–32 μg/ml and 2–10 μg/ml, respectively. Fourier transform infrared spectroscopic analysis of EPS showed the presence of carbohydrates, proteins, alkyl halides, and nucleic acids. X-ray diffraction analysis revealed crystalline structure of EPS extracted from biofilm forming bacteria. These findings suggest a high prevalence of antibiotic-resistant bacteria in orthopaedic implant-associated surgeries, highlighting the urgent need for ongoing monitoring and microorganism testing in infected implants.


Introduction
Orthopaedic implants in recent years have revolutionized the treatment of health related issues such as osteoarthritis and other distressing problems, for example bone fractures [1,2].The rate of infections during surgical operations has increased despite the availability of better procedure options and strategies [3][4][5].Several researchers suggested in their findings that the breach to skin barriers during the surgical operations, exposed the body to various bacterial infections [6][7][8].After the insertion of the orthopaedic implant, the inevitable deposition of the host body proteins onto the implant device forms a favourable environment for the microorganisms to colonize and develop into biofilms [4,9].These biofilms on the orthopaedic implants protect the bacteria from antimicrobial treatments, such as antibiotics, therefore limiting treatment options, which further result in poor clinical outcomes [10,11].Once developed into biofilms, the bacteria are very difficult to treat because of the greater resistance to conventional antibiotics.These bacteria also further evade the host defence systems [12][13][14][15].Such infections pose further pressure on health systems by prolonged stays in health-care facilities, increased prescribing of antimicrobial agents-such as antibiotics-and laboratory costs, amongst many others [16][17][18][19][20].
Of the bacterial species identified in the orthopaedic implant infections, the Gram-positive Staphylococci are the most prevalent species.Different studies showed that Staphylococcus aureus accounts for 20-30% of infections whereas the coagulase-negative Staphylococci are responsible for approximately 20-40% of surgical implant infections.Other less frequent microbes identified in the orthopaedic implant-associated infections include the Gram-positive cocci, such as Streptococci and Enterococci, as well as Gram-negative bacilli, for example Pseudomonas aeruginosa and the Enterobacteriaceae family [21][22][23][24].
This study aimed to isolate and characterize bacteria from post-surgical orthopaedic implant infections as well as assess antibiotic resistance profiles, explore the capacity for biofilm formation, and elucidate the role of extracellular polymeric substances (EPS) in shaping both antibiotic resistance and the development of biofilms.

Ethical approval statement
The ethical review committee of the Fauji Foundation Hospital and Fatima Jinnah Women University have thoroughly reviewed the study.The committees did not find anything in the study which was against the ethical guidelines of biomedical research involving animals and human subjects.

Sample collection, isolation, and identification of strains
The samples for this cross-sectional study were collected from orthopaedic departments at three hospitals: Fauji Foundation Hospital and Pakistan Atomic Energy Commission Hospital in Rawalpindi, and Kulsum International Hospital in Islamabad.In this study, we focused only on the culturable bacterial diversity present in the collected samples, therefore excluding nonculturable diversity.When a patient with an infected orthopaedic implant was admitted, the hospital team notified researchers of the surgery schedule.Surgeons provided discarded skin, deep tissue, and explanted prosthesis samples during surgery.These samples were immediately transferred to sterilized tubes containing 0.9% saline and then to the Microbiology and Biotechnology Research Laboratory at Fatima Jinnah Women University within an hour.The samples were then inoculated into brain heart infusion (BHI) broth, and grown aerobically for 24 hours at 37˚C.Serial dilutions of the culture were prepared, and single bacterial colonies were obtained following serial dilution and streaking.The pure culture was preserved in Luria Bertani (LB) glycerol stock solution at -80˚C.Overall, 44 distinct colonies were isolated, the morphology of the isolated colonies was studied with the naked eye as well as under microscopes.Gram staining procedure was applied to study the cell morphology and then biochemical characterization was carried out using API 10 S strips [25].

16S rRNA gene sequencing analysis
The 16S rRNA gene, approximately 1500 bp long with 9 hypervariable regions (V1 -V9) was used for the identification of eleven strains [28].The bacterial isolates were identified using the V3 and V4 hypervariable regions in the 16S rRNA gene.Genomic DNA was extracted from the bacteria following the protocol described by Vingataramin and Frost [29].Colony PCR was carried out to amplify the 16S rRNA-encoding gene.Briefly, 250 ng of bacterial DNA was used as a template with 0.5 μM (each) primer (341F [5-CCTAYGGGRBGCASCAG-3] and 806R [5-GGACTACNNGGGTATCTAAT-3]), 200 μM deoxynucleoside triphosphate, and 2 U of Taq high-fidelity DNA polymerase (Boehringer Mannheim) in a 1× amplification buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl, 1.5 mM MgCl 2 ).The PCR conditions consisted of denaturation of the mixture at 94˚C for 2 minutes, the mixture was then subjected to 40 cycles of annealing at 56˚C for 1 minute, 1 min of elongation at 72˚C, and 1 min of denaturation at 94˚C.A final extension step was achieved at 72˚C for 10 minutes.PCR products were resolved by electrophoresis in a 1% agarose gel stained with ethidium bromide.The sequences obtained were verified online using the NCBI gene bank, http://www.ncbi.nlm.nih.gov/.After obtaining accession numbers, MEGA X software was used to generate a phylogenetic tree.

Production and extraction of extracellular polymeric substances (EPS)
Fresh culture of bacterial strains was used for extraction of the EPS.The production of extracellular polymeric substances was carried out using EPS medium [30].Flasks containing 50 mL of the EPS broth were inoculated with 1 mL of the bacterial suspension.Flasks were placed in a shaking incubator at 150 rev min -1 , at 37 ˚C for 72 hour.The cells were collected through sedimentation (10,000 rev min-1) at 4 ˚C and for 15 min.The extracted EPS was obtained from the subsequent supernatant, which was precipitated using prechilled acetone (1:2) and kept at 4˚C overnight.After spinning the mixture down at 10,000 rev min-1 for 15 min, the collected pellets were dried in a desiccator for further analysis.
Quantification and characterization of EPS.Protein content in the EPS was quantified as per the Bradford method [31].The phenol sulfuric acid method was used for the quantification of total polysaccharides in the EPS [32].Absorption was measured at 490 nm and the concentrations were determined using a standard curve of glucose.The main functional groups present in the EPS of the selected bacterial were identified via Fourier transform infrared spectroscopy (FTIR-8400 Schimadzu).Briefly, dried EPS samples were mixed with spectroscopic grade potassium bromide (KBr) in a 5:95 ratio.Pellets of the samples were prepared using a hydraulic presser.Infrared spectrum in the 4000-400 cm -1 region with 15 scan speed was generated using a spectrophotometer [33].X-ray diffraction (XRD) of the powdered EPS was performed using copper-Kα x-rays at a wavelength of 1.5406 Å. 2θ data was obtained at a range from ~5˚to 80˚, with a scan step size of 0.02˚step and time per step of 0.4 s with voltage of 40 Kv and 30 mA beam current [34].

Bacterial cell adhesion to the hydrocarbon (BATH) test
Hydrophobic surface characteristics of bacterial cells were determined using the BATH test [35].Briefly, the bacterial cells were cultured until the logarithmic phase in nutrient broth.The cells were pelleted and washed twice with a phosphate urea magnesium (PUM) buffer.The bacterial cells, normalized to an OD 400 of 1.0 (1.2 mL), were transferred to a series of clean test tubes, to which hexadecane was added.The mixture in the test tubes was thoroughly mixed for 15 minutes.The test tubes were then left for 2 minutes for phase separation.Optical density of the aqueous phase was measured at 400 nm using an UV-visible spectrophotometer.PUM buffer was used as reference.

Microtiter plate assay
Microtiter plate assay was used to observe the biofilm forming ability of bacterial strains [36].An overnight culture was pelleted and washed with phosphate buffered saline (PBS).The culture suspension was then diluted to an OD 600 of 0.05.An aliquot (100 μL volume) of the diluted culture was added to each well in a 96-well microtiter plate.The plate was then sealed with a sterile gas-permeable film and incubated without shaking at 37˚C for 24 hours.After this time, the attached biomass was quantified by crystal violet staining.Briefly, the culture in each well was removed by aspiration.The wells were washed three times with deionized water (200 μL) and air-dried.The attached biomass was then stained by the addition of 0.1% w/v crystal violet for 10 min.Following this, the stain was removed by aspiration and the wells were washed three times with deionized water (200 μL) and airdried.The residual crystal violet stain associated with the attached biomass was solubilized by adding 200 μL of acetic acid (30% v/v) in each well.After 10 minutes the OD 595 was measured spectrophotometrically.A well containing sterile LB served as a negative control.

Statistical analysis
Results of each experiment were stated as a mean of three technical replicate ± standard deviation (SD).One-way ANOVA with Fisher Pairwise Comparison was used for evaluating the biofilm formation capacity, extracellular polymeric substance production, and adhesion of the bacterial cell to hydrocarbon.Correlation analysis of hydrophobicity of the bacteria and EPS formation was determined by Principal Component Analysis (PCA) using Minitab 19 software.The clades were formed based on the similarity of biochemical tests.Strains having similar results were clustered together using PAST software version 3.12.

Patient history and Sample collection
Samples were collected from 16 patients between 11-83 years old over the period of 1 year from different hospitals in Islamabad and Rawalpindi.All the patients were identified with post-operative infections.Risk factors that are associated with orthopaedic implant infections, include diabetes, hypertension, obesity, and older age.Patients with diabetes are at a higher risk of infection compared to other health related issues.The results indicated that 31.25% of the patients admitted for the revision surgery had either diabetes or pre-diabetes, whereas 18.75% had hypertension.The meta data of the clinical bacterial isolates obtained from discarded skin and a deep tissue samples as well as infected explanted prostheses provided by the operating surgeons is shown in Table 1.

Isolation of strains and morphological characteristics
Colony morphology of bacterial isolates was examined in terms of colony appearance.All colonies were circular in shape, having raised elevation and smooth margins, except for MB647, which had a wrinkled appearance.Out of forty-four isolates, seventeen bacterial isolates were cocci, whereas the rest belonged to group bacilli (S1 Table ).Most strains were capsule formers.Cells of different isolates exhibited various arrangements, while some bacteria formed filaments (S2 Table ).All the isolates were mesophiles and showed growth at the range of pH 7-9.Isolates MB633, MB635, MB637, MB638, MB663, MB664, MB665, MB666, MB667 and MB668 had mucilaginous, while the rest had a creamy texture.

Antibiotic resistance profile
Bacterial strains showed resistance to most antibiotics used in the study.Bacterial isolates MB641 showed sensitivity to polymyxin only, and exhibited resistance to all other antibiotics Multiple antibiotic resistance (MAR) index.Strains were classified as sensitive, intermediate, and resistant to antibiotics, based on the size of the zone of inhibition.Multiple antibiotic resistance (MAR) computed for each strain showed high values.Multiple antibiotic resistance pattern in bacterial strains isolated from surgical implant infections was also studied.The majority of the bacterial strains exhibited resistance to the selected antibiotics.The overall trend indicated that MB642 and MB655 were the most resistant bacteria with a MAR index of 0.9, whereas MB643, MB646 and MB652 also exhibited multiple resistance against approximately 80% of antibiotics used, with a MAR index of 0.88.Isolates MB648, MB649, MB631,

16S rRNA gene sequencing analysis
After initial screening, including morphological, biochemical, and antibiotic resistance profiling, 11 bacterial strains were selected for 16S rRNA gene sequencing and further study.The identity of the screened strains was established using 16S rRNA gene sequencing and subsequent comparison of the output sequence data against the NCBI database http://www.ncbi.nlm.nih.gov/.The query cover, percentage identity, and closest match information for each strain can be found in Table 2 along with the corresponding accession numbers generated during the analysis.This information provides a comprehensive overview of the taxonomic classification and closest matches of the selected strains in our study.Phylogenetic analysis was carried out to determine the similarity with other bacterial strains of the same genus (Fig 3).

Correlation between EPS production and hydrophobicity
The EPS production and relative hydrophobicity of the bacterial strains was assessed.The polysaccharides and protein content of the EPS were found to be in the range of 11-32 μg/ml and 2-10 μg/ml, respectively.Correlation between the EPS production and hydrophobicity in clinical isolates was assessed after 24 hours of incubation.Results indicated a positive correlation between hydrophobicity and EPS production except in Bacillus spp.MB647 that showed no tendency towards hydrocarbons adhesion.Bacillus spp.MB647 also produced less extracellular polymeric substances as compared to other bacterial strains but highest biofilm formation ability.The highest hydrophobicity was observed in five Pseudomonas strains i.e., MB633,

Identification of the functional groups
In the present study, the EPS matrix was extracted to study different functional groups present in it, such as protein, humic substances, and polysaccharides, along with other substances.The position and number of FTIR peaks for the EPS fractions seemed to be relatively close, suggesting that the nature of chemical groups in these fractions were similar (Fig 5).Numerous strong frequency band linked with proteins and polysaccharides were readily observed in the EPS components.The results showed predominant spectral bands for hydroxyl (OH) at 3600-3200 cm −1 , amide (NH) at 2970-2850 cm −1 , weak peak of carbonyl (-COOR) at 1740 cm − 1 , alkoxyl ester (RO) at 1255 cm − 1 , carbonyl (C OC) at 1167 cm − 1 , and acetal linkage (O-C-O) at 1039 cm − 1 [37,38].

X-ray diffraction (XRD)
X-ray diffraction profile of EPS was carried out to study the nature of the extracellular polymeric substance.All the selected samples showed characteristic sharp peaks at 24.10˚, 30.91å nd 46.67˚indicating crystalline and the well-developed nature of the compound (Fig 6).

Biofilm formation ability
PAO1 was used as a reference strain for studying biofilm producing strains.PAO1 is a wellstudied strain and a known biofilm former, which makes it a good model for studying biofilm formation in other bacteria [39].The biofilm forming ability of the identified strains was evaluated using the classic crystal violet assay after 24 hours of incubation.Within the spectrum of bacterial strains investigated, Bacillus spp.MB647 exhibited the highest biofilm-forming ability, with a value of 0.55 ± 0.06.Notably, Pseudomonas spp.strains MB663 and MB664 also demonstrated significant biofilm-forming potential, with values of 0.54 ± 0.01 and 0.52 ± 0.02 respectively, after the 24-hour incubation period.The strains that exhibited the lowest biofilmforming abilities were Enterobacter cloacae MB649, with a value of 0.3 ± 0.006, and Planococcus chinensis MB653, which showed the lowest value of 0.22 ± 0.004.The remaining strains displayed biofilm formation within the range of 0.42 to 0.48 (as shown in Fig 7).These findings provide insights into the varying biofilm-forming capacities of the bacterial strains under study after the specified 24-hour incubation period.
Fisher pairwise comparison with one way ANOVA significantly depicted p = 0.000 high biofilm formation in all the tested strains except for MB653 and MB649.Both of these strains have significantly p < 0.05 low biofilm producing abilities which are validated by statistical analysis.Although the biofilm formation capacity of all the strains was good, the comparison

Discussion
The microbiological diagnosis of orthopaedic implant-associated infections is vital for effective management.These infections are a major concern for patients and can lead to prolonged hospitalization, increased healthcare costs, and even the need for revision surgery, especially in developing countries [40].This study focused on the phenotypic variation and the antibiotic resistance pattern in the causative pathogens isolated from infected orthopaedic implant samples.Its findings highlight a significant rate of antibiotic resistance in bacterial isolates obtained from 16 surgical implant infections in a one-year sampling period.Initially, bacterial isolates were identified through morphological and biochemical characterization methods.Various bacterial strains were successfully isolated and identified in this present study, such as Staphylococcus aureus, as well as multidrug-resistant Gram-negative rods such as E. coli, Klebsiella pneumonia, and Pseudomonas aeruginosa.According to a study by Moftian et al. the prevalence of antibiotic-resistant Gram-negative bacteria, such as E. coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, is highest in Asia and in the Middle East, ranging from 30% to 80% depending on the antibiotic used [41].A study that analysed the global distribution and transmission of carbapenem-resistant Enterobacteriaceae (CRE) suggested that South Asia has a high frequency of carbapenem-resistant Enterobacteriaceae [42].Other studies mentioned coagulase-negative Staphylococci, S. aureus, Enterococcus species and E.coli from such similar infections [43,44].In this current study, the antibiotic resistance pattern of the 44 clinical isolates was investigated.The MAR index was used to identify the resistance pattern in the clinical isolates.The MAR index � 0.2 indicates high resistance and increased use of antibiotics in that specific area [45].Several studies have looked into the MAR index (MARI) of bacteria obtained from samples of human tissue.In 2016, a study examined data from patients in India and discovered that the average MARI was greater than 0.2, indicating evidence of antibiotic resistance [46].A study conducted in Pakistan showed multi-resistance in 157 (81.3%) bacterial strains, while 162 strains had a multi-antibiotic resistance index (MAR) of 0.2.[47].Muhindo et al. examined patients with surgical site infections in Uganda and reported an average MARI of 0.6, signifying substantial antibiotic resistance [48].Our study revealed a significant MAR index of 0.6.Furthermore, the results indicated that over 90% of isolates had a MAR index of 0.2.This high level of antibiotic resistance presents clinicians with the challenge of limited treatment choices.Multidrug resistance further narrows available options for antibiotic therapy [49][50][51].
After the initial assessment, eleven bacterial isolates were selected for identification via 16S rRNA gene sequencing based on their physiological, biochemical characterization, and antibiotic resistance profiles.Production of extracellular polymeric substance (EPS) of the selected strains was assessed and quantified.It is a well-known fact that EPS play a key role in the attachment as well as colonization of bacteria on surfaces, including implant surfaces, and also provide a physical barrier that protect the bacteria from the host immune system.It was observed by researchers that some bacterial isolates have been found to be both more hydrophobic and produce more EPS [52].Researchers suggested that the amount of EPS required to form mature colonies by different bacteria, depend upon genus or species [53].
In the current study, maximum EPS production as well as good biofilm film forming ability and hydrophobicity was observed in all Pseudomonas aeruginosa strains.This finding is in line with previous research suggesting that Pseudomonas species require greater EPS production, particularly for primary adhesion to substrates [54].This combination of characteristics makes them particularly well suited to form biofilms on implant surfaces and can lead to increased resistance to antibiotics which was observed in the bacterial strains isolated from the samples.These bacteria have been found to form biofilms on the implant surface, allowing them to evade the host immune response and antibiotics [55][56][57][58].Studies have shown that EPS contribute to antibiotic resistance through various mechanisms.A key method involves limiting antibiotic penetration via the polysaccharide matrix [59].Biofilm EPS shields bacteria from the host's complement system, reducing immune response and promoting survival [60,61].Protective EPS in mixed bacterial biofilms helps non-resistant bacteria endure antibiotics and even transfer resistance genes [61].Research shows exopolysaccharide degrading enzymes (EPDEs) enhance antibiotic efficacy, as seen with P. aeruginosa treated with ciprofloxacin and EPDE alginate lyase in mouse models [62,63].
Various functional groups within the EPS matrix were investigated in the current research, including proteins, humic substances, polysaccharides, and other components, using FTIR spectroscopy.The analysis confirmed the presence of proteins, humic substances, and nucleic acids in the samples [64,65].The characteristic peaks, indicative of amide and carboxylic groups, were consistent across all EPS samples and aligned with prior studies [66,67].It is evident from the comparative spectra of selected samples of EPS matrices that they possess similarity in terms of their chemical composition and proportion of chemical constituents.Xray diffraction technique was applied for phase identification of extracellular polymeric substance.The XRD diffractograms showed predominantly sharp peaks that indicate predominance of the crystallinity and uniform crystal lattice, as shown in previous literature [68].The results are encouraging in terms of high crystallinity which gives a strong three dimensional architecture of biofilms thereby acting as a reinforcing grid to form strong multicellular entities [69,70].
The results of the present study showed that the detection of orthopaedic implant-associated infections through microbiological testing is critical in treating complex cases and preventing further complications.For the purpose of creating efficient treatment plans, it is essential to identify the bacterial species and patterns of antibiotic resistance in implant infection samples.The significant rate of antibiotic resistance discovered in this study emphasises the urgent requirement for new, affordable medicines to treat bacterial surgical site infections.Hence, more investigation is needed to find new therapeutic modalities that can supplement or lessen the need of antibiotics.In addition to helping patients, this will also contribute to addressing the growing issue of antibiotic resistance in hospital settings.

Conclusion
Antibiotic resistance has become a global issue and is causing high mortality rates worldwide.This study indicates that the menace of MDR pathogens is prevalent in Pakistan because of the indiscriminate and excessive use of antibiotics without appropriate investigation and physician's knowledge.It is a well-known fact that the introduction of a foreign object into its host, such as an orthopaedic device, would increase the susceptibility of infection.The management of these orthopaedic device-associated infections represents a great challenge to health practitioners and researchers.The results of this study provides better insight in understanding the role of various virulence factors that may have a role in the colonization and the adaptation of the etiological agents in the host.

Fig 1 .
Fig 1. Clustering of the bacterial isolates obtained from the infected tissue samples based on various biochemical characters.Similarity among isolates was assessed by conducting 10 different biochemical sugar fermentation tests using the API 10 S strips and converting them into binary data, 0 for negative and 1 for positive test results, respectively, using PAST (Paleontological Statistics Software Package for Education and Data Analysis software).Similarities amongst the strains were estimated using the Jacquard coefficient, and the unweighted average linkage gave the cluster.Consequently, bacterial isolates sharing the greatest similarity are clustered together, enabling a clear visualization of their relatedness.https://doi.org/10.1371/journal.pone.0292956.g001

Fig 2 .
Fig 2. The graph depicting the bacterial species' MAR index from samples of infected tissues.The MAR index is indicative of the level of resistance of the isolated bacterial isolates.The multiple antibiotic resistance (MAR) indices were determined with reference to the tested antibiotics, and it was above 0.2 in almost 90% of the bacteria studied.Highest MAR index was observed in MB655 (0.88) and MB649 (0.8) while lowest MAR index was observed in bacterial strain MB645 (0.14) and MB651 (0.15).https://doi.org/10.1371/journal.pone.0292956.g002

Fig 3 .
Fig 3. Phylogenetic tree of dominant bacterial diversity isolated from infected soft tissues.Evolutionary trees were constructed in MEGAX software by using Neighbor-Joining (NJ) method.Bootstrap values (expressed as percentages of 1000 replications) are shown at the branch points.Clinical isolates studied in present research are highlighted with a red dot.Eleven morphological distinct strains were selected, and the sequences obtained were aligned with their closest reference bacterial sequences available in GenBank using Basic Local Alignment Search Tool (BLAST) for identification indicated in the figure mentioned above.https://doi.org/10.1371/journal.pone.0292956.g003

Fig 4 .
Fig 4. Correlation between the Extracellular polymeric substances production and hydrophobicity of selected isolates.The release of extracellular polymeric substance and relative hydrophobicity of the clinical isolates after 24 hours of incubation is shown in the figure above.A positive correlation can be observed between most strains.Strain 647 showed no tendency to adhesion toward hydrocarbon.Depicting the inability of the strain for adherence to hydrophobic surface.Data represents average of three technical replicates ± SD. https://doi.org/10.1371/journal.pone.0292956.g004

Fig 5 .Fig 7 .
Fig 5. FTIR Spectra of selected EPS matrix obtained from different bacterial strains.The figure represents the Fourier transform infrared spectroscopic analysis of EPS of different pure-culture bacteria.The figure shows the major functional groups present in the EPS.Infrared spectra of bacterial EPS indicated the alcohols, Carboxylic acids, esters, alkyl halides, and alkenes functional groups.The figure is prepared in Originpro 8.5 software.https://doi.org/10.1371/journal.pone.0292956.g005